| |
REAGENT/ACTION |
: DESCRIPTION |
* |
Detection of Urine Sugars |
|
A |
Benedicts reagent |
Sodium Citrate 170 g
Sodium Carbonate anhydrous 100 g
Copper sulphate 17 g
To prepare 1.0 Litre solution |
* |
Detection of Urine Proteins |
|
A |
20% Sulphasalicylic acid |
Sulphosalicylic acid 100 g. To prepare 500 ml solution. |
* |
Detection of Urine Bilirubin |
|
B |
(i) 10% Barium chloride |
Barium chloride 50 g. To prepare 500 ml solution. |
B |
(ii) Fouchet's reagent |
10% Ferric chloride - 100 ml & 25% Trichloroacetic acid - 900 ml |
* |
Detection of Urine Ketone bodies |
|
B |
Rothera's reagent |
Sodium nitroprusside 3 g
Ammonium Sulphate 100 g
Sodium carbonate 50 g. The mixture store in a screw capped bottle |
* |
Stool examination (wet prep) |
|
A |
Physiological saline |
Sodium chloride 9 g
To prepare 1.0 litre solution |
* |
Blood examination for parasites |
|
A |
Field stain B |
Field stain B - 10 g To prepare 1.0 litre solution. |
* |
Blood examination (Haemoglobin) |
B |
Drabkin's solution |
Potassium cyanide 0.1 g
Potassium ferricyaride 0.4 g
Sodium bicarbonate 2.0 g
To prepare 1.0 litre solution. |
* |
Skin disinfection |
|
A |
(i) 70% Alcohol |
Alcohol - 700 ml
Gentian violet - 1 drop
Distilled water - 300 mL |
| |
(ii) Rectified spirit |
Ready made |
* |
Examination by microscope |
|
A |
Immersion oil |
|
* |
BACTERIA STAINING |
|
| |
I. Gram staining |
|
B |
(i) Gentian violet |
Gentian violet 5 g
To prepare 1.0 litre solution |
B |
(ii) Lugols Iodine |
Iodine 10 g
Potassium iodide 20 g.
To prepare 1.0 litre solution. |
B |
(iii) 10% Acetone/Alcohol |
Acetone 100 ml & Ethanol 900 ml. |
B |
(iv) Neutral red |
Neutral red chloride 1 g
1% Acetic acid - 1 mL
To prepare 1.0 litre solution |
II. |
Zn staining |
|
B |
(i) Basic fuchsin |
10 g basic fuchsin in 100 ml of pure methanol |
B |
(ii) 20% Sulphuric acid |
200 ml concentrated Sulphuric acid to prepare 1 litre of solution. |
B |
(iii) Methylene blue |
Methylene blue 10 g
To prepare 1.0 litre solution. |
* |
Fungal identification |
|
C |
Potassium hydroxide 20% |
Potassium hydroxide 20 g to prepare 100 ml solution. |
* |
Tissue fixation |
|
B |
(i) 10% Formal - saline |
Formaldehyde 40% - 100 ml
Sodium chloride 9 g to prepare 1.0 litre solution. |
C |
(ii) 50/50 Ether Alcohol |
500 Ether plus 500 ml Ethyl alcohol to prepare 1 litre. |
* |
Transport media |
|
B |
Stuart's transport media |
Stuarts transport Medium - 8 g
To prepare 500 ml.
Distribute into bijou bottles, screw caps on securely sterilize, label and store after cooling. |
* |
Detection of Typhoid/Typhus fever |
B |
(i) Salmonella H suspension |
Vial 5 ml |
B |
(ii) Salmonella 0 x 19 suspension |
Vial 5 ml |
B |
(iii) Proteus 0 x 19 suspension |
Vial 5 ml |
* |
Detection of Brucellosis |
|
B |
Brucella arbutus suspension |
Vial 5 ml |
| |
Pregnancy detection |
Vial 5 ml |
C |
Piano sec |
Kit |
* |
Syphil's screening |
|
C |
V.D.R.L reagent |
Kit |
* |
HIV screening |
|
C |
HIV check |
Kit |
* |
Blood grouping |
|
C |
Anti A serum |
Vial 10 ml |
C |
Anti B serum |
Vial 10 ml |
C |
Anti A,B Serum (O serum) |
Vial 10 ml |
C |
Anti D serum |
Vial 5 ml |
* |
Crossmatch test |
|
C |
30% Burive albumin |
Vial 30 ml |
C |
Coomb's reagent |
Vial 2 ml |
C |
Incomplete ant D |
Vial 5 ml |
* |
Sickling test |
|
A |
2% Sodium metabisulphite |
Sodium Metabisulphite 2%
To prepare fresh 5 mL solution |
* |
Total white Blood cell count |
|
C |
Turk's fluid |
Acetic acid glacial 2% & 1 drop gentian violet to prepare 100 ml. |
* |
Blood glucose determination |
Detection of Diabetics |
c |
(i) 5% Trichloroacetic acid |
Trichloroacetic acid 50 g. to prepare 1.0 litre solution. |
| |
(ii) O - Toluidine reagent |
Thiourea 1.5 g
Acetic acid glacial
950 ml & O - toluidine 50 mL. |
c |
(iii) Glucose standard |
Glucose pure 18 g
Benzoic acid 1%
To prepare 1.0 litre stock solution. |
* |
Detection of Renal problems: |
|
| |
creatinine determination |
|
C |
(i) 5% Trichloroacetic acid |
as for glucose |
C |
(ii) 0.6% Picric acid |
saturated
picric acid 250 mL of Distilled water 250 mL. |
C |
(iii) 1.0 N Sodium hydroxide |
Sodium hydroxide 40 g
To prepare 1.0 litre solution. |
C |
(iv) 0.1 N Hydrochloric acid |
Hydrochloric acid 8 ml to prepare 1.0 litre solution |
C |
(v) Creatinine standard |
Creatinine crystals 0.1 g
To prepare 1.0 litre
stock solution with 0.1 N Hydrochloric acid. |
* |
Detection of Jaundice. |
|
* |
Bilirubin determination |
|
C |
(i) Sulfanilic acid |
Sulfanilic acid 0.2 g, conc Hcl 1.5 ml. To prepare 100 ml. |
C |
(ii) Sodium nitrite |
Sodium nitrite 0.2 g to prepare 100 ml solution. |
C |
(iii) Caffeine reagent |
caffeine 50 g, Sodium benzoate 5 g,
Sodium acetate 125 g, EDTA 1 g. To prepare 1.0 litre solution |
C |
(iv) Tartrate solution |
Potassium Sodium tartrate 250 g,
Sodium hydroxide 20 g to prepare 1.0 litre solution. |
C |
(v) Bilirubin standard |
Commercial serum-made |
C |
(vi) Saline |
Sodium chloride 9 g. To prepare 1.0 litre solution |
